Products/Services Used | Details | Operation |
---|---|---|
Gene Synthesis> | To generate the HOTTIP repair template, a sequence harboring the exonic portion of HOTTIP, flanked by 352- and 237-bp mouse homologous arms, was synthesized and cloned into puc19 using the SmaI restriction enzyme site (GenScript). To generate the murine HDR template a sequence harboring the endogenous UMLILO promoter and the exonic portion of UMLILO flanked by 500-bp murine homologous arms was synthesized and cloned into puc19 (GenScript). | Get A Quote |