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Staphylococcus aureus Leukocidins Target Endothelial DARC to Cause Lethality in Mice.

Cell Host Microbe. 2019-03; 
LubkinAshira,LeeWarren L,AlonzoFrancis,WangChangsen,AligoJason,KellerMatthew,GirgisNatasha M,Reyes-RoblesTamara,ChanRita,O'MalleyAidan,BuckleyPeter,VozhillaNikollaq,VasquezMarilyn T,SuJohnny,SugiyamaMichael,YeungStephen T,CoffreMaryaline,BajwaSofia,ChenEric,MartinPatricia,KimSang Y,LoomisCynthia,WorthenG Scott,ShopsinBo,KhannaKamal M,WeinstockDaniel,LynchAnthony Simon,KoralovSergei B,LokeP'ng,CadwellKen,TorresVict
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Molecular Biology Reagents Purified leukocidins were tested for LPS with ToxinSensor Chromogenic LAL Endotoxin Assay Kit (GenScript) and found to contain less than 0.006 EU/ml in the maximum amount used for injections. Get A Quote

摘要

The pathogenesis of Staphylococcus aureus is thought to depend on the production of pore-forming leukocidins that kill leukocytes and lyse erythrocytes. Two leukocidins, Leukocidin ED (LukED) and γ-Hemolysin AB (HlgAB), are necessary and sufficient to kill mice upon infection and toxin challenge. We demonstrate that LukED and HlgAB cause vascular congestion and derangements in vascular fluid distribution that rapidly cause death in mice. The Duffy antigen receptor for chemokines (DARC) on endothelial cells, rather than leukocytes or erythrocytes, is the critical target for lethality. Consistent with this, LukED and HlgAB injure primary human endothelial cells in a DARC-dependent manner, and mice wi... More

关键词

ACKR1,DARC,HlgAB,LukED,Staphylococcus aureus,endothelial cells,leukoc