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Induction of a Spindle-Assembly-Competent M Phase in Xenopus Egg Extracts.

Curr Biol. 2019-04; 
BishtJitender S,TomschikMiroslav,GatlinJes
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摘要

Normal mitotic spindle assembly is a prerequisite for faithful chromosome segregation and unperturbed cell-cycle progression. Precise functioning of the spindle machinery relies on conserved architectural features, such as focused poles, chromosome alignment at the metaphase plate, and proper spindle length. These morphological requirements can be achieved only within a compositionally distinct cytoplasm that results from cell-cycle-dependent regulation of specific protein levels and specific post-translational modifications. Here, we used cell-free extracts derived from Xenopus laevis eggs to recapitulate different phases of the cell cycle in?vitro and to determine which components are required to rend... More

关键词

Greatwall kinase,M phase,Xenopus egg extract,cell cycle,chromokinesin,cyclin,mitosis,spindle asse