Tumor-infiltrating human CD4 regulatory T cells display a distinct TCR repertoire and exhibit tumor and neoantigen reactivity.

CD4 regulatory T (T) cells have an essential function in maintaining self-tolerance; however， they may also play a detrimental role in antitumor immune responses. The presence of elevated frequencies of T cells in tumors correlates with disease progression and poor survival in patients with cancer. The antigen specificity of T cells that have expanded in the tumor microenvironment is poorly understood; answering this question may provide important insights for immunotherapeutic approaches. To address this， we used a novel combinatorial approach to characterizing the T cell receptor (TCR) profiles of intratumoral T cells from patients with metastatic melanoma， gastrointestinal， and ovarian cancers and elucidated their antigen specificities. The TCR repertoires of tumor-resident T cells were diverse yet displayed significant overlap with circulating T cells but not with conventional T cells in tumor or blood. TCRs isolated from T cells displayed specific reactivity against autologous tumors and mutated neoantigens， suggesting that intratumoral T cells act in a tumor antigen-selective manner leading to their activation and clonal expansion in the tumor microenvironment. Tumor antigen-specific T-derived TCRs resided in the tumor and in the circulation， suggesting that both T cell compartments may serve as a source for tumor-specific TCRs. These findings provide insights into the TCR specificity of tumor-infiltrating human T cells that may have potential implications for cancer immunotherapy.