| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | The sequence of the truncated human PDE5A1 (hPDE5) was obtained by gene synthesis in the pUC57 vector (GenScript) and digested with SmaI and NheI enzymes (New England Biolabs). | Get A Quote |
cGMP is critical to a variety of cellular processes， but the available tools to interfere with endogenous cGMP lack cellular and subcellular specificity. We introduce SponGee， a genetically encoded chelator of this cyclic nucleotide that enables in?vitro and in?vivo manipulations in single cells and in biochemically defined subcellular compartments. SponGee buffers physiological changes in cGMP concentration in various model systems while not affecting cAMP signals. We provide proof-of-concept strategies by using this tool to highlight the role of cGMP?signaling in?vivo and in discrete subcellular domains. SponGee enables the investigation of local cGMP signals in?vivo and paves the way for therapeutic stra... More
