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Molecular characterization and pathogenicity of Francisella noatunensis subsp. orientalis isolated from cultured tilapia (Oreochromis sp.) in Taiwan.

J. Fish Dis.. 2019-05; 
PulpipatTheeraporn,LinKuo-Hua,ChenYing-Hao,WangPei-Chi,ChenShih
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Molecular Biology Reagents … 0.01 M EDTA, and 1 M boric acid). The gels were stained with DNAsafe™ Nucleic Acid Gel Stain II (GenScript, USA) and visualized under UV light using the Gel Doc™ XR+ (Bio‐rad, Canada). Bands of the size 1,150 bp were … Get A Quote

摘要

Francisella noatunensis subsp. orientalis is a causative agent of systemic granulomatous disease in tilapia. The present study was designed to understand the genetic and phenotypic diversities among Taiwanese Fno isolates obtained from tilapia (n = 17) and green Texas cichlid (Herichthys cyanoguttatus) (n = 1). The enzymatic profiles of the isolates were studied using the API ZYM system. Phylogenetic tree analysis of the 16S rRNA and housekeeping gene and pulsed-field gel electrophoresis (PFGE) were carried out to determine the genotypic characters of all isolates. The phylogenetic tree showed similarity of 99%-100% nucleotide sequences of 16S rRNA and housekeeping genes compared to the Fno references genes... More

关键词

Francisella noatunensis susbsp. orientalis,Oreochromis sp,PFGE,genotype,pathogenicity,pheno