A novel prokaryotic vector for identification and selection of recombinants: direct use of the vector for expression studies in E. coli.

The selection of bacterial recombinants that harbour a desired insert， has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screening high number of colonies， generation of false positives， setting up of control ligation mix with vector alone etc.