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A common polymorphism within MSLN affects miR-611 binding site and soluble mesothelin levels in healthy people.

J Thorac Oncol. 2014;

GarritanoSonia，De SantiChiara，SilvestriRoberto，MelaiuOmbretta，CipolliniMonica，BaroneElisa，LucchiMarco，BaraleRoberto，MuttiLuciano，GemignaniFederica，BonottiAlessandra，FoddisRudy，CristaudoAlfonso，LandiSte

Products/Services Used	Details	Operation
Custom Vector Construction	… The 3′UTR of MSLN (NM_005823.5) bearing the G allele of rs1057147 was cloned downstream the luciferase gene into pmiRGLO vector (Promega, Madison, WI) by GenScript Company (Piscataway, NJ) using SacI and XhoI as restriction enzymes at 5′ and 3′ end …	Get A Quote

摘要

Soluble mesothelin related peptide (SMRP) was proposed as a promising diagnostic marker for malignant pleural mesothelioma (MPM). In a previous study， we found that rs1057147 within the 3' untranslated region of MSLN gene was associated with SMRP levels. Thus， we aimed to (1) confirm the previous association on a large series of volunteers and (2) test the hypothesis that the SNP could affect microRNA binding sites.