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CO₂directly modulates connexin 26 by formation of carbamate bridges between subunits.

Elife. 2013; 
MeighLouise,GreenhalghSophie A,RodgersThomas L,CannMartin J,RoperDavid I,DaleNich
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Custom Vector Construction … Materials and methods Hemichannel expression and mutagenesis All connexin genes except Cx26R104A, Cx26K125E and Cx26R104E were synthesised by Genscript USA and subcloned into the pCAG-GS mCherry vector … Get A Quote

摘要

Homeostatic regulation of the partial pressure of CO2 (PCO2) is vital for life. Sensing of pH has been proposed as a sufficient proxy for determination of PCO2 and direct CO2-sensing largely discounted. Here we show that connexin 26 (Cx26) hemichannels, causally linked to respiratory chemosensitivity, are directly modulated by CO2. A 'carbamylation motif', present in CO2-sensitive connexins (Cx26, Cx30, Cx32) but absent from a CO2-insensitive connexin (Cx31), comprises Lys125 and four further amino acids that orient Lys125 towards Arg104 of the adjacent subunit of the connexin hexamer. Introducing the carbamylation motif into Cx31 created a mutant hemichannel (mCx31) that was opened by increases in ... More

关键词

connexins,membrane channels,respiratory chemosensitivity,signal transduc