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| Molecular Biology Reagents | … The PCR was performed in a Mastercycler® (Eppendorf) using Taq polymerase (Genscript, NJ) at a concentration of 1u/25µl reaction consisted of 2 min incubation at 94C followed by 30 cycles of 15 sec denaturation at 94C, 15 sec extension at 55C and 1 min … | Get A Quote |
Soybean vein necrosis-associated virus has been linked to an emerging soybean disease in the United States and Canada. Virus distribution and population structure in major growing areas were evaluated. Data were employed to design and develop sensitive detection protocols， able to detect all virus isolates available in databases. The host range for the virus was assessed and several species were found to sustain virus replication， including ivyleaf morning glory， a common weed species in soybean-growing areas in the United States. Koch's postulates were fulfilled using soybean thrips and transmission efficiency was determined. This article provides significant insight into the biology of the most widespre... More
