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Perfluorooctane sulfonate disturbs Nanog expression through miR-490-3p in mouse embryonic stem cells.

PLoS ONE. 2013; 
XuBo,ChenXiaojiao,MaoZhilei,ChenMinjian,HanXiumei,DuGuizhen,JiXiaoli,ChangChunxin,RehanVirender K,WangXinru,XiaYa
Products/Services Used Details Operation
Custom Vector Construction The 3′UTR sequence of Nanog predicted to interact with miR-490-3p or a mutated sequence with the predicted target sites were inserted into the KpnI and SacI sites of pGL3 promoter vector (Genscript, Nanjing, China). Get A Quote

摘要

Perfluorooctane sulfonate (PFOS) poses potential risks to reproduction and development. Mouse embryonic stem cells (mESCs) are ideal models for developmental toxicity testing of environmental contaminants in vitro. However, the mechanism by which PFOS affects early embryonic development is still unclear. In this study, mESCs were exposed to PFOS for 24 h, and then general cytotoxicity and pluripotency were evaluated. MTT assay showed that neither PFOS (0.2 µM, 2 µM, 20 µM, and 200 µM) nor control medium (0.1% DMSO) treatments affected cell viability. Furthermore, there were no significant differences in cell cycle and apoptosis between the PFOS treatment and control groups. However, we found... More

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