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Metabolic engineering of microbes for branched-chain biodiesel production with low-temperature property.

Biotechnol Biofuels. 2015;

TaoHui，GuoDaoyi，ZhangYuchen，DengZixin，LiuTian

Products/Services Used	Details	Operation
Gene Synthesis	The alsS gene [GenBank: 936852] from B. subtilis was synthesized using GenScript (Nanjing, China) with the sequence of the ribosome binding site (rbs) and cloned into the XbaI-BamHI sites of the plasmid pET28a (+), yielding pDG16. The ilvC [GenBank: 948286] and ilvD [GenBank: 948277] genes were amplified from E. coli genomic DNA with primers ilvC-XbaI/ilvC-SpeI-BamHI and ilvD-XbaI/ilvD-SpeI-BamHI, and the PCR products were cloned into pET28a (+), yielding pDG17 and pDG18, respectively.	Get A Quote

摘要

The steadily increasing demand for diesel fuels calls for renewable energy sources. This has attracted a growing amount of research to develop advanced， alternative biodiesel worldwide. Several major disadvantages of current biodiesels are the undesirable physical properties such as high viscosity and poor low-temperature operability. Therefore， there is an urgent need to develop novel and advanced biodiesels.