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Inhibition of BmNPV replication in silkworm cells using inducible and regulated artificial microRNA precursors targeting the essential viral gene lef-11.

Antiviral Res.. 2014; 
ZhangJun,HeQian,ZhangChun-Dong,ChenXiang-Yun,ChenXue-Mei,DongZhan-Qi,LiNa,KuangXiu-Xiu,CaoMing-Ya,LuCheng,PanMin
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Custom Vector Construction AmiRNA se431 quences with flanking sequences were synthesized and cloned into 432 a pUC57-simple vector by GenScript (Nanjing, China). Get A Quote

摘要

Bombyx mori nucleopolyhedrovirus (BmNPV) is a major silkworm pathogen, causing substantial economic losses to the sericulture industry annually. We demonstrate a novel anti-BmNPV system expressing mature artificial microRNAs (amiRNAs) targeting the viral lef-11 gene. The mature amiRNAs inhibited the lef-11 gene in silkworm BmN-SWU1 cells. Antiviral assays demonstrated that mature amiRNAs silenced the gene and inhibited BmNPV proliferation efficiently. As constitutive overexpression of mature amiRNAs may induce acute cellular toxicity, we further developed a novel virus-induced amiRNA expression system. The amiRNA cassette is regulated by a baculovirus-induced fusion promoter. This baculovirus-induced RNA in... More

关键词

Artificial microRNA,Baculovirus-induced RNA interference system,BmNPV,RNA interfer