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RNA interference‑mediated knockdown of translationally controlled tumor protein induces apoptosis, and inhibits growth and invasion in glioma cells.

Mol Med Rep. 2015; 
JinHua,ZhangXuexin,SuJun,TengYueqiu,RenHuan,YangLizh
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Custom Vector Construction .1 eukaryotic expression vector (GenScript, Nanjing, China) using the restriction sites of HindIII and BamHI, and the resulting plasmid was termed pRNA-H1.1-TCTP. Get A Quote

摘要

Translationally controlled tumor protein (TCTP) is a highly conserved, growth‑associated and small molecule protein, which is highly expressed in various types of tumor cell. TCTP can promote the growth and suppress apoptosis of tumor cels. However, few studies have reported the effects of TCTP in gliomas. In the present study, a glioma cell line was established, which was stably transfected with TCTP short hairpin ribonucleic acid (shRNA), to investigate the impact of downregulated expression of TCTP on the proliferation, apoptosis and invasion of glioma cells. Western blot and reverse transcription-quantitative polymerase chain reaction analyses demonstrated that TCTP shRNA effectively reduced... More

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