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| Gene Synthesis | qPCR were then performed. Primers specific for P. waltl and murine C3 as well as housekeeping transcripts (Table 1) were designed using the Genscript software (http://genscript.com/ssl-bin/app/primer) in different exons to ensure that they could not hybridize to potential traces of genomic DNA. | Get A Quote |
The complement system plays an important role in inflammation， innate and acquired immunity， as well as homeostasis. Despite these functions， the effects of spaceflight conditions on the complement system have not yet been intensively studied. Consequently， we investigated the effects of five types of chronic stressors， similar to those encountered during a stay onboard the International Space Station， on C3 expression in larvae of the urodele amphibian . We focused on C3 because it is a critical component of this system. These studies were completed by the analysis of adult mice exposed to two models of inflight stressors. Our data show that simulating space radiation， or combining a modification... More
