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The characteristics and expression profiles of the mitochondrial genome for the Mediterranean species of the Bemisia tabaci complex.

BMC Genomics. 2013; 
WangHua-Ling,YangJiao,BoykinLaura M,ZhaoQiong-Yi,LiQian,WangXiao-Wei,LiuShu-S
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Custom Vector Construction Long PCRs were carried out using LA Taq DNA polymerase with the following PCR conditions: 95°C for 2 min, followed by 35 cycles of 96°C for 30 s, 48-56°C for 30 s, 72°C for 3 min and a final cycle of 72°C for 10 min. PCR fragments were purified and ligated into the pGEM-T Easy Vector (Promega, USA) and sequenced in both directions using the ABI BigDye 3.1 at GenScript (Nanjing, China). Get A Quote

摘要

The whiteflies under the name Bemisia tabaci (Gennadius) (Aleyrodidae: Hemiptera) are species complex of at least 31 cryptic species some of which are globally invasive agricultural pests. Previously, the mitochondrial genome (mitogenome) of the indigenous New World B. tabaci species was sequenced and major differences of gene order from the postulated whitefly ancestral gene order were found. However, the sequence and gene order of mitogenomes in other B. tabaci species are unknown. In addition, the sequence divergences and gene expression profiles of mitogenomes in the B. tabaci species complex remain completely unexplored.

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