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Engineering the esaR promoter for tunable quorum sensing- dependent gene expression.

ACS Synth Biol. 2013; 
ShongJasmine,CollinsCynth
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Custom Vector Construction The PesaR‑D, C, P promoters (Figure S1, Supporting Information) were generated by DNA synthesis (GenScript) and were provided in a pUC57 vector. Get A Quote

摘要

Quorum sensing (QS) systems enable bacteria to coordinate their behavior as a function of local population density and are often used in synthetic systems that require cell−cell communication. We have engineered the esaR promoter, P(esaR), which is repressed by the QS regulator E(saR). E(saR)-dependent gene expression from P(esaR) is induced by 3-oxo-hexanoyl-homoserine lactone (3OC6HSL). Here, we report a set of modified P(esaR) promoters that contain a second E(saR) binding site. We observed changes in gene expression levels, regulatory range, 3OC6HSL sensitivity, and the regulatory role of E(saR) that are dependent on the position of the second binding site. Combining the new promoters with end... More

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