Alterations in microRNAs expression can accelerate the development of human cancers. However， the role of miR-153-3p in acute lymphoblastic leukemia remains unknown. The expression of miR-153-3p in acute lymphoblastic leukemia cell lines was measured by quantitative real-time polymerase chain reaction. Effects of miR-153-3p expression on acute lymphoblastic leukemia cell proliferation， migration， and invasion were examined by Cell Counting Kit-8 assay， wound healing assay， and Transwell invasion assay， respectively. We then validated inhibitor of growth protein 2 as a direct target of miR-153-3p through bioinformatics analysis， luciferase activity reporter assay， and Western blot assay. The miR-... More
Alterations in microRNAs expression can accelerate the development of human cancers. However， the role of miR-153-3p in acute lymphoblastic leukemia remains unknown. The expression of miR-153-3p in acute lymphoblastic leukemia cell lines was measured by quantitative real-time polymerase chain reaction. Effects of miR-153-3p expression on acute lymphoblastic leukemia cell proliferation， migration， and invasion were examined by Cell Counting Kit-8 assay， wound healing assay， and Transwell invasion assay， respectively. We then validated inhibitor of growth protein 2 as a direct target of miR-153-3p through bioinformatics analysis， luciferase activity reporter assay， and Western blot assay. The miR-153-3p expression was decreased in acute lymphoblastic leukemia cell lines. Cell proliferation， migration， and invasion of acute lymphoblastic leukemia were obviously decreased by miR-153-3p overexpression. Moreover， inhibitor of growth protein 2 was validated as a direct target of miR-153-3p and the overexpression of inhibitor of growth protein 2 reversed the suppressive effects of miR-153-3p on acute lymphoblastic leukemia cell behaviors. Based on these results， we provided evidence that miR-153-3p might be a target for the treatment of acute lymphoblastic leukemia.
