Traditional strategies for establishing shRNA expression constructs are inefficient， error-prone， or costly. We describe a simple approach that overcomes these drawbacks. Briefly， the sense and antisense strands of the short hairpin RNA coding sequence are segmented into two parts， respectively， at asymmetric sites. The four resulting short oligonucleotides are synthesized. Each oligonucleotide is annealed with its opposite， resulting in a double-stranded fragment with sticky termini at both ends. The two fragments so generated can be easily spliced by simple ligation to reconstitute the full-length short hairpin RNA coding sequence which can then be cloned into an appropriately restricted vector.
Traditional strategies for establishing shRNA expression constructs are inefficient， error-prone， or costly. We describe a simple approach that overcomes these drawbacks. Briefly， the sense and antisense strands of the short hairpin RNA coding sequence are segmented into two parts， respectively， at asymmetric sites. The four resulting short oligonucleotides are synthesized. Each oligonucleotide is annealed with its opposite， resulting in a double-stranded fragment with sticky termini at both ends. The two fragments so generated can be easily spliced by simple ligation to reconstitute the full-length short hairpin RNA coding sequence which can then be cloned into an appropriately restricted vector.
