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Kir2.1 and K2P1 channels reconstitute two levels of resting membrane potential in cardiomyocytes.

J. Physiol. (Lond.). 2017; 
ZuoDongchuan,ChenKuihao,ZhouMin,LiuZheng,ChenHa
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Custom Vector Construction … Grundy (2015). Constructs and adenoviruses. The human sequence encoding Kir2.1 (AF153820) was synthesized and cloned into the pUC57 vector (GenScript Corporation, Piscataway, NJ, USA) at the EcoRV site. The inserts … Get A Quote

摘要

Outward and inward background currents across the cell membrane balance, determining resting membrane potential. Inward rectifier K channel subfamily 2 (Kir2) channels primarily maintain the resting membrane potential of cardiomyocytes. Human cardiomyocytes exhibit two levels of resting membrane potential at subphysiological extracellular K concentrations or pathological hypokalaemia, however, the underlying mechanism is unclear. In the present study, we show that human cardiomyocytes derived from induced pluripotent stem cells with enhanced expression of isoform 1 of Kir2 (Kir2.1) channels and mouse HL-1 cardiomyocytes with ectopic expression of two pore-domain K channel isoform 1 (K2P1) recapitulate t... More

关键词

K2P1 channels,Kir2.1 channels,resting membrane poten