Dysregulation of long non-coding RNAs (lncRNAs) confirm that it plays a crucial role in tumourigenesis and malignant progression of glioma. The present study demonstrated that LncRNA secretory carrier membrane protein 1 (SCAMP1) was up-regulated and functioned as an oncogene in glioma cells. In addition， miR-499a-5p was down-regulated meanwhile exerted tumour-suppressive function in glioma cells. Subsequently， inhibition of SCAMP1 significantly restrained the cell proliferation， migration and invasion， as well as promoted apoptosis by acting as a molecular sponge of miR-499a-5p. Transcription factor LIM homeobox transcription factor 1， alpha (LMX1A) was overexpressed in glioma tissues and cells. Moreo... More
Dysregulation of long non-coding RNAs (lncRNAs) confirm that it plays a crucial role in tumourigenesis and malignant progression of glioma. The present study demonstrated that LncRNA secretory carrier membrane protein 1 (SCAMP1) was up-regulated and functioned as an oncogene in glioma cells. In addition， miR-499a-5p was down-regulated meanwhile exerted tumour-suppressive function in glioma cells. Subsequently， inhibition of SCAMP1 significantly restrained the cell proliferation， migration and invasion， as well as promoted apoptosis by acting as a molecular sponge of miR-499a-5p. Transcription factor LIM homeobox transcription factor 1， alpha (LMX1A) was overexpressed in glioma tissues and cells. Moreover， miR-499a-5p targeted LMX1A 3'-UTR in a sequence-specific manner. Hence， down-regulation of SCAMP1 remarkably reduced the expression level of LMX1A， indicating that LMX1A participated in miR-499a-5p-induced tumour-suppressive effects on glioma cells. Furthermore， knockdown of LMX1A decreased NLR family， CARD domain containing 5 (NLRC5) mRNA and protein expression levels through directly binding to the NLRC5 promoter region. Down-regulation of NLRC5 obviously inhibited malignant biological behaviours of glioma cells through attenuating the activity of Wnt/β-catenin signalling pathway. In conclusion， our study clarifies that SCAMP1/miR-499a-5p/LMX1A/NLRC5 axis plays a critical role in modulating malignant progression of glioma cells， which provide a novel therapeutic strategy for glioma treatment.
