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Evaluation of the AID AmpC line probe assay for molecular detection of AmpC producing Enterobacterales.

J Glob Antimicrob Resist. 2019; 
WagnerKaroline,ManciniStefano,RitterClaudia,BöttgerErik C,KellerPet
Products/Services Used Details Operation
Plasmid DNA Preparation … chromosomal ampC sequences with mutations in the promoter (bimut, -15 ln t, -15 ln tg) and attenuator regions (mut2, del2) that were not available from clinical Enterobacterales isolates were synthesized and cloned into the pUC57-plasmid by Genscript Biotech. Corp … Get A Quote

摘要

In this study, the commercially available AID AmpC line probe assay (LPA) was evaluated for detection of plasmid-mediated ampC genes in Enterobacterales and chromosomal mutations in the ampC promoter/attenuator regions in Escherichia coli.

关键词

AmpC β-lactamases,Enterobacterales,Molecular detection,antibiotic resist