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SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of L-phenylalanine aldolase.

Biotechnol. Lett.. 2019; 
WangYida，TianJunwei，XiaoYing，WangYue，SunHongxu，ChangYanhong，Lu
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Plasmid DNA Preparation … All other reagents were of analytical grade. Plasmid construction. The LPA gene encoding L-phenylserine aldolase (GenBank: BAD91543.1) was synthesized by GenScript (Nanjing, China) and inserted into the pET28a vector between the sites of BamHI and HindIII … Get A Quote

摘要

To improve the thermostability and organic solvent tolerance of L-phenylserine aldolase， the in vivo SpyTag/SpyCatcher cyclization strategy was applied in this work.

关键词

L-phenylserine aldolase，Organic solvent tolerance，Protein engineering，SpyTag/SpyCatcher，Thermostabi
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