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Tankyrase-1 Ankyrin Repeats Form an Adaptable Binding Platform for Targets of ADP-Ribose Modification.

Structure. 2016; 
EisemannTravis,McCauleyMichael,LangelierMarie-France,GuptaKushol,RoySwati,Van DuyneGregory D,PascalJo
Products/Services Used Details Operation
Custom Vector Construction All other TNKS fragments were expressed from a synthetic TNKS gene (Genscript) in a pET47 expression vector...All other peptides were purchased from Genscript or EZBiolab Get A Quote

摘要

The poly(ADP-ribose) polymerase enzyme Tankyrase-1 (TNKS) regulates multiple cellular processes and interacts with diverse proteins using five ankyrin repeat clusters (ARCs). There are limited structural insights into functional roles of the multiple ARCs of TNKS. Here we present the ARC1-3 crystal structure and employ small-angle X-ray scattering (SAXS) to investigate solution conformations of the complete ankyrin repeat domain. Mutagenesis and binding studies using the bivalent TNKS binding domain of Axin1 demonstrate that only certain ARC combinations function together. The physical basis for these restrictions is explained by both rigid and flexible ankyrin repeat elements determined in our structural analy... More

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