| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … After the ligation of adapters, 5 μl of digested/ligated DNA were pre-amplified in 25 μl reaction containing 2 μl each of pre-amplification primers (8 pmol/μl), 0.2 mMdNTPs, 1X PCR buffer (Genscript, USA), and 1 unit of Taq DNA polymerase (Genscript, USA) (5 units/μl) … | Get A Quote |
Herbicide resistant (HR) rice improves efficiency of weed management and HR varieties can develop through induced mutagenesis. The present investigation was focused on mutagenic response of seed-derived rice calli to different EMS concentrations and evaluation for HR. Seed-derived calli were obtained from glyphosate-susceptible rice variety (Bg250) and exposed to varying concentrations of EMS (0.1%, 0.2%, 0.3% and 0.4%). EMS treated calli were exposed to glyphosate (0.2%) and Tetrazolium test (1% TTC) was applied to identify cell viability in calli. Fluorescent AFLP analysis was performed on EMS treated calli. Results indicated that mutation of calli was higher at EMS concentration range of 0.1 – 0.2%. Bg... More
