| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … the Weizmann Institute. 2.2. Induction and evaluation of EAE. EAE was induced in (SJL/JxBALB/c)F1 mice by the peptide encompassing amino acids 139–151 of proteolipid protein (PLP) synthesized by Genscript. Mice were … | Get A Quote |
The lack of biomarkers is a major obstacle for investigating myelin repair. We used metabolic incorporation of the choline analog - propargyl-choline (P-Cho) to label and visualize newly synthesized myelin in the CNS of mice induced with experimental autoimmune encephalomyelitis (EAE). We further developed unbiased colocalization analysis to quantify P-Cho incorporation specifically into the myelin. Our findings indicate that P-Cho injection to mice recovering from EAE， either spontaneously or following glatiramer acetate treatment， results in significant elevation of its incorporation into the myelin， offering a novel strategy for assessing remyelination in animal models and the remyelination potential o... More
