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Dual-Stage Crosslinking of a Gel-Phase Bioink Improves Cell Viability and Homogeneity for 3D Bioprinting.

Adv Healthc Mater. 2016-11;

DubbinKaren，HoriYuki，LewisKazuomori K，HeilshornSar

Products/Services Used	Details	Operation
Peptide Synthesis	… Experimental Section. Material Synthesis: P1 peptide (EYPPYPPPPYPSGC, 1536 g mol −1 ) was purchased from Genscript Corp (Piscataway, NJ, USA) and Alginate was purchased from Novamatrix (Sandvika, Norway) in the LVG formulation (MW 75–200 kDa) …	Get A Quote

摘要

Current bioinks for cell-based 3D bioprinting are not suitable for technology scale-up due to the challenges of cell sedimentation， cell membrane damage， and cell dehydration. A novel bioink hydrogel is presented with dual-stage crosslinking specifically designed to overcome these three major hurdles. This bioink enables the direct patterning of highly viable， multicell type constructs with long-term spatial fidelity.