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A new class of fatty acid allene oxide formed by the DOX-P450 fusion proteins of human and plant pathogenic fungi, C. immitis and Z. tritici.

J. Lipid Res.. 2016-09; 
OliwErnst H,AragóMarc,ChenYang,JernerénFre
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Custom Vector Construction … Sequencing was performed at Uppsala Genome Center (Uppsala University). The open reading frames of GenBank EAS28473 (C. immitis), EGP83657 (Z. tritici), and EGP87976 (Z. tritici) in pUC57 vectors were purchased from GenScript (Piscataway, NJ) … Get A Quote

摘要

Linoleate dioxygenase-cytochrome P450 (DOX-CYP) fusion enzymes are common in pathogenic fungi. The DOX domains form hydroperoxy metabolites of 18:2n-6, which can be transformed by the CYP domains to 1,2- or 1,4-diols, epoxy alcohols, or to allene oxides. We have characterized two novel allene oxide synthases (AOSs), namely, recombinant 8R-DOX-AOS of Coccidioides immitis (causing valley fever) and 8S-DOX-AOS of Zymoseptoria tritici (causing septoria tritici blotch of wheat). The 8R-DOX-AOS oxidized 18:2n-6 sequentially to 8R-hydroperoxy-9Z,12Z-octadecadienoic acid (8R-HPODE) and to an allene oxide, 8R -epoxy-9,12Z-octadecadienoic acid, as judged from the accumulation of the α-ketol, 8S-h... More

关键词

8-dioxygenase,Coccidioides immitis,Zymoseptoria tritici,cyclooxygenase,cytochrome P450 74 family,dioxygenase,lipid metabolism,lipidomics,oxygenation mecha