| Products/Services Used | Details | Operation |
|---|---|---|
| CRISPR Bacteria & Yeast Engineering | … Stable cell lines expressing HRAS were selected for 10 days with 0.5 μg/ml puromycin 48 h after infection. HRAS expression in MKN-28 cells was knocked down using siRNA. The HRAS and non-targeted RNA were obtained from GenScript (Nanjing, China) … | Get A Quote |
Gastric carcinoma is one of the most lethal malignancies of cancers and its prognosis remains dismal due to the paucity of effective therapeutic targets. Herein， we showed that HRAS is markedly up-regulated in gastric carcinoma. Prognostic analysis indicated that HRAS expression might be a prognostic indicator for the survival of patients with gastric carcinoma. Ectopic expression of HRAS in gastric carcinoma cells accelerated proliferation， migration， invasion， angiogenesis， and clone formation ability of gastric carcinoma cells in vitro. Furthermore， HRAS over-expressing significantly promoted the tumorigenicity of gastric carcinoma cells in vivo whereas silencing endogenous HRAS caused opposite o... More
