Partially acetylated chitosan oligosaccharides (paCOS) are potent biologics with many potential applications， and their bioactivities are believed to be dependent on their structure， i.e.， their degrees of polymerization and acetylation， as well as their pattern of acetylation. However， paCOS generated via chemical N-acetylation or de-N-acetylation of GlcN or GlcNAc oligomers， respectively， typically display random patterns of acetylation， making it difficult to control and predict their bioactivities. In contrast， paCOS produced from chitin deacetylases (CDAs) acting on chitin oligomer substrates may have specific patterns of acetylation， as shown for some bacterial CDAs. However， compare... More
Partially acetylated chitosan oligosaccharides (paCOS) are potent biologics with many potential applications， and their bioactivities are believed to be dependent on their structure， i.e.， their degrees of polymerization and acetylation， as well as their pattern of acetylation. However， paCOS generated via chemical N-acetylation or de-N-acetylation of GlcN or GlcNAc oligomers， respectively， typically display random patterns of acetylation， making it difficult to control and predict their bioactivities. In contrast， paCOS produced from chitin deacetylases (CDAs) acting on chitin oligomer substrates may have specific patterns of acetylation， as shown for some bacterial CDAs. However， compared to what we know about bacterial CDAs， we know little about the ability of fungal CDAs to produce defined paCOS with known patterns of acetylation. Therefore， we optimized the expression of a chitin deacetylase from the fungus Puccinia graminis f. sp. tritici in Escherichia coli The best yield of functional enzyme was obtained as a fusion protein with the maltose-binding protein (MBP) secreted into the periplasmic space of the bacterial host. We characterized the MBP fusion protein from P. graminis (PgtCDA) and tested its activity on different chitinous substrates. Mass spectrometric sequencing of the products obtained by enzymatic deacetylation of chitin oligomers， i.e.， tetramers to hexamers， revealed that PgtCDA generated paCOS with specific acetylation patterns of A-A-D-D， A-A-D-D-D， and A-A-D-D-D-D， respectively (A， GlcNAc; D， GlcN)， indicating that PgtCDA cannot deacetylate the two GlcNAc units closest to the oligomer's nonreducing end. This unique property of PgtCDA significantly expands the so far very limited library of well-defined paCOS available to test their bioactivities for a wide variety of potential applications.
