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Transgenic plants over-expressing insect-specific microRNA acquire insecticidal activity against Helicoverpa armigera: an alternative to Bt-toxin technology

Transgenic Research. 2015; 
Aditi Agrawal, Vijayalakshmi Rajamani, Vanga Siva Reddy, Sunil Kumar Mukherjee, Raj K. Bhatnagar
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Custom Vector Construction … 1) Both pre-amiR-24 and pre-amiR-24C (229 bp) were synthesized commercially (Genscript, USA) and cloned individually into a pUC57 vector as an expression cassette at XbaI/SacI restriction sites Open image in new window Fig 1 Fig 1 … Get A Quote

摘要

The success of Bt transgenics in controlling predation of crops has been tempered by sporadic emergence of resistance in targeted insect larvae. Such emerging threats have prompted the search for novel insecticidal molecules that are specific and could be expressed through plants. We have resorted to small RNA-based technology for an investigative search and focused our attention to an insect-specific miRNA that interferes with the insect molting process resulting in the death of the larvae. In this study, we report the designing of a vector that produces artificial microRNA (amiR), namely amiR-24, which targets the chitinase gene of Helicoverpa armigera. This vector was used as transgene in tobacco. Northern b... More

关键词

Artificial miRNA; Agrobacterium; Transgenic tobacco; Insecticidal; Insect bioassay