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Multiple conformations are a conserved and regulatory feature of the RB1 5' UTR.

RNA. 2015; 
KutchkoKatrina M,SandersWes,ZiehrBen,PhillipsGabriela,SolemAmanda,HalvorsenMatthew,WeeksKevin M,MoormanNathaniel,LaederachA
Products/Services Used Details Operation
Custom Vector Construction The mutant sequences, which varied only by point mutations, were inserted into pUC57 by Genscript. sequences were also cloned by Genscript into pUC57.For luciferase assays, each RB1 construct was cloned into the pGL3-control vector between the SV40 promoter and the Firefly luciferase CDS by Genscript. Get A Quote

摘要

Folding to a well-defined conformation is essential for the function of structured ribonucleic acids (RNAs) like the ribosome and tRNA. Structured elements in the untranslated regions (UTRs) of specific messenger RNAs (mRNAs) are known to control expression. The importance of unstructured regions adopting multiple conformations, however, is still poorly understood. High-resolution SHAPE-directed Boltzmann suboptimal sampling of the Homo sapiens Retinoblastoma 1 (RB1) 5' UTR yields three distinct conformations compatible with the experimental data. Private single nucleotide variants (SNVs) identified in two patients with retinoblastoma each collapse the structural ensemble to a single but distinct well-defin... More

关键词

SHAPE,UTR,covariation,retinoblastoma,riboSN