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Custom Vector Construction> | The P0-ECD construct that contained the 124 amino acid residues (aa 1–124) from the extracellular domain (ECD) of rat P0 was cloned and expressed using the expression vector C5 (GenScript, Piscataway, NJ) or pET23d (+)( EMD Millipore, Billerica MA) as described previously . ...The final endotoxin level was reduced to <1 EU/µg using the ToxinEraser Endotoxin Removal kit (Genscript, Piscataway, NJ). ...The stimulation index was defined by cpm in the presence of antigen divided by cpm in the absence of antigen. P0 peptide (180–199) was purchased from Genscript (Piscataway, NJ). | Get A Quote |
Spontaneous autoimmune polyneuropathy (SAP) in B7-2 knockout NOD mice mimics the progressive form of chronic inflammatory demyelinating polyradiculoneuropathy, and is mediated by myelin protein zero (P0)-reactive Th1 cells. In this study, we focused on the effect of B7-2 deletion on the function of dendritic cells (DCs) within the context of SAP. We found that development of SAP was associated with a preponderance or increase of CD11b(+) DCs in peripheral lymph nodes and sciatic nerves. B7-2 deletion led to altered immunophenotypic properties that differ between CD11b(+) DCs and CD8α(+) DCs. Both DC subsets from B7-2 knockout NOD mice exhibited impaired capacity to capture fluorophore-labeled myelin P0, ... More