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Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus.

Virus Res.. 2015; 
WuAndong,WangYi,ZengCong,HuangXingyu,XuShan,SuCeyang,WangMin,ChenYu,GuoD
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Custom Vector Construction … 22 Construction of recombinant 3CLpro expression vectors The coding sequence of MERS-CoV nsp5 (NC_019843) was synthesized chemically by GenScript and cloned into vectors pET28a and pGEX-6p-1, respectively … Get A Quote

摘要

Coronavirus 3C-like protease (3CLpro) is responsible for the cleavage of coronaviral polyprotein 1a/1ab (pp1a/1ab) to produce the mature non-structural proteins (nsps) of nsp4-16. The nsp5 of the newly emerging Middle East respiratory syndrome coronavirus (MERS-CoV) was identified as 3CLpro and its canonical cleavage sites (between nsps) were predicted based on sequence alignment, but the cleavability of these cleavage sites remains to be experimentally confirmed and putative non-canonical cleavage sites (inside one nsp) within the pp1a/1ab awaits further analysis. Here, we proposed a method for predicting coronaviral 3CLpro cleavage sites which balances the prediction accuracy and false positive outcomes. ... More

关键词

3C-like protease,Canonical cleavage sites,MERS-CoV,Michaelis constants,Non-canonical cleavage s