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MIR144 and MIR451 regulate human erythropoiesis via RAB14.

Br. J. Haematol.. 2015-02; 
KimMinJung,TanYee Sun,ChengWen-Chih,KingsburyTami J,HeimfeldShelly,CivinCu
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Custom Vector Construction … The gene enoding ZHER2:2891-ABD-PE38X8 with the N-terminal amino acid sequence HEHEHE and (S4G)3 linkers connecting the three domains in the expression vector pET-26b(+) (Merck) was obtained from GenScript USA Inc (Piscataway, NJ, USA) … Get A Quote

摘要

Expression levels of MIR144 and MIR451 increase during erythropoiesis, a pattern that is conserved from zebrafish to humans. As these two miRs are expressed from the same polycistronic transcript, we manipulated MIR144 and MIR451 in human erythroid cells individually and together to investigate their effects on human erythropoiesis. Inhibition of endogenous human MIR451 resulted in decreased numbers of erythroid (CD71(hi) CD235a(hi) CD34(-) ) cells, consistent with prior studies in zebrafish and mice. In addition, inhibition of MIR144 impaired human erythroid differentiation, unlike in zebrafish and mouse studies where the functional effect of MIR144 on erythropoiesis was minimal. In this study, we ... More

关键词

MIR144,MIR451,RAB14,erythropoietic inhibitor,human erythropoi