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Nanoparticle-based lateral flow biosensor for visual detection of fish nervous necrosis virus amplification products.

Mol. Cell. Probes. 2015-06; 
ToubanakiDimitra K,MargaroniMaritsa,KaragouniEvd
Products/Services Used Details Operation
Custom Vector Construction … primer (DpInRGNdv) specific for the RGNNV genotype Their sequences are summarized in Table 1 In order to optimize tetra-primer PCR, two reference plasmids were constructed by GenScript (Piscataway, NJ) and used as targets … Get A Quote

摘要

Lateral flow paper biosensors are an attractive analytical platform for detection of human and veterinary disease pathogens because they are optimal for accurate, rapid and sensitive analysis in research laboratory setups, as well as field analysis. Since diseases of viral etiology have been wreaking havoc in aquaculture industry, as well as the environment, the present study aims at the development of a gold nanoparticle-based biosensor for fish nervous necrosis virus (Nodavirus) nucleic acids detection. Total viral RNA, isolated from fish samples was subjected to reverse transcription PCR amplification. The PCR products were mixed with a specific oligonucleotide probe and applied next to oligonucleo... More

关键词

Dicentrarchus labrax,Gold nanoparticles,Lateral flow strip biosensor,Nervous necrosis virus,Nodavirus,RT