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Effect of sodium/iodide symporter (NIS)-mediated radioiodine therapy on estrogen receptor-negative breast cancer.

Oncol. Rep.. 2015; 
YaoChen,PanYi,LiYongxin,XuXiangdong,LinYing,WangWenjian,WangShen
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Peptide Synthesis … nanomaterials of defined structure and controllable function Experimental Procedures Peptide Synthesis Peptides were either purchased from GenScript USA (Piscataway, NJ) or synthesized in-house In the latter case, peptides were … Get A Quote

摘要

Since the sodium/iodide symporter (NIS) stimulates the iodine uptake in normal lactating breast, our study aimed to study the effect of NIS-mediated radioiodide therapy on ER-negative breast cancers. A recombinant lentivirus plasmid encoding the human NIS (hNIS) gene and firefly luciferase (Fluc) was constructed. MDA-MB-231 cells were transfected with the recombinant lentivirus, and the hNIS gene expression was identified by western blot analysis and real-time PCR. Tissue-specific expression of the NIS gene was confirmed by immunohistochemical (IHC) staining. Functional NIS activity in the MDA-hNIS cells was confirmed by the uptake of 131I and cytotoxicity assays. The relative expression level of hNIS mRNA ... More

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