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Agrobacterium tumefaciens-mediated transformation of the entomopathogenic fungus Nomuraea rileyi.

Fungal Genet. Biol.. 2015; 
ShaoChangwen,YinYouping,QiZhaoran,LiRen,SongZhangyong,LiYan,WangZhong
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Custom Vector Construction … 22, 24] The DNA fragment of the SFFV promoter/enhancer was synthesized by GenScript (Piscataway, NJ, http://wwwgenscriptcom), based on the DNA sequence of the pSFFV‐neo‐BCL‐XL expression vector [24] Third, the … Get A Quote

摘要

An Agrobacterium-mediated genetic transformation system for the entomopathogenic fungus Nomuraea rileyi was established. Three binary T-DNA vectors, pPZP-Hph, pPZP-Hph-RNAi and pPZP-Hph-DsRed2, were constructed. The trpc promoter from Aspergillus nidulans was used as the cis-regulatory element to drive the expression of hygromycin phosphotransferase (hph) gene and DsRed2, which conferred the hygromycin B (Hyg B) resistance and red fluorescence visualization, respectively. The blastospores and conidia were used as the recipients. The blastospores' transformation efficiency reached ∼20-40 transformants per 10 blastospores, whereas the conidia were not transformed. Based on an analysis of five gener... More

关键词

Agrobacterium tumefaciens-mediated transformation (ATMT),Hygromycin phosphotransferase gene (hph),Nomuraea rileyi,Red fluorescent gene (DsRed2),Vector construc