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Custom Vector Construction> | … the shuttle vector pRS415 The high-level, constitutive Ashbya gossypii TEF promoter was obtained from pRH499 (GenScript, Piscataway, NJ, USA) The A gossypii TEF promoter region was from −1 to −379 [43] The [P TEF … | Get A Quote |
Triacetic acid lactone (TAL) is a potential platform chemical that can be produced in yeast. To evaluate the potential for industrial yeast strains to produce TAL, the g2ps1 gene encoding 2-pyrone synthase was transformed into 13 industrial yeast strains of varied genetic background. TAL production varied 63-fold between strains when compared in batch culture with glucose. Ethanol, acetate, and glycerol were also tested as potential carbon sources. Batch cultures with ethanol medium produced the highest titers. Therefore, fed-batch cultivation with ethanol feed was assayed for TAL production in bioreactors, producing our highest TAL titer, 5.2 g/L. Higher feed rates resulted in a loss of TAL and su... More