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Strain-specific interaction of a GII.10 Norovirus with HBGAs.

Virology. 2015-02; 
JinMiao,TanMing,XiaMing,WeiChao,HuangPengwei,WangLeyi,ZhongWeiming,DuanZhaojun,Jia
Products/Services Used Details Operation
Custom Vector Construction … The P domain-encoding cDNA sequences of Vietnam 026 (GII10) (GenBank Accession noAF504671, amino acid sequences from 222 to 557 of VP1 were used) were synthesized (GenScript, Piscataway, NJ) and cloned into the expression vector pGEX-4T-1 (GE Healthcare … Get A Quote

摘要

Noroviruses (NoVs), an important cause of gastroenteritis in humans, recognize human histo-blood group antigens (HBGAs) as receptors. The crystal structures of the protruding (P) domain of a GII.10 NoV (Vietnam 026) in complex with various HBGA oligosaccharides were elucidated. However, the HBGA binding profile of this virus remains unknown. In this study, we determined the saliva and oligosaccharide binding profiles of this virus and the roles of amino acids that are involved in HBGA binding. Our data showed that Vietnam 026 bound to all ABO secretor and non-secretor saliva with clear signals detected by monoclonal antibodies against H3, H1, Le(y), Le(a) and sialyl Le(a). Mutagenesis study confir... More

关键词

Acute gastroenteritis,Human histo-blood group antigens,Mutagenesis,Noroviruses,Oligosaccharide,Sa