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Custom Vector Construction> | … To construct HDAC1 targeting siRNA expression vectors, DNA fragments encoding short hairpin RNA targeting HDAC1 were inserted into the vector pRNA-CMV31/Neo (GenScript, Piscataway, NJ, USA) between the Bam HI and Hind III sites … | Get A Quote |
Plasmid-cured derivative strains of Bacillus anthracis are frequently used in laboratory studies. Plasmid incompatibility, which does not increase the risk of chromosomal mutation, is a useful method for plasmid curing. However, in bacteria containing multiple plasmids, it often requires the sequential introduction of multiple, specific incompatibility plasmids. This lengthy process renders the traditional plasmid incompatibility method inefficient and mutation-prone. In this study, we successfully cured plasmids pXO1 and pXO2 from B. anthracis A16 simultaneously using only one recombinant incompatible plasmid, pKORT, to obtain a plasmid-free strain, designated A16DD. This method may also be u... More