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The ATC/TTC haplotype in the Interleukin 8 gene in response to Gram-negative bacteria: a pilot study

Archives of Oral Biology. 2019-07; 
Suzane C. Pigossi Giovana Anovazzi Livia S. Finoti Marcell C. de Medeiros Tatian Maria De Souza Moreira Marcia P.A.Mayer Cleslei Fernando ZanellicSandro Robert Valentinic Carlos Ross Junior Raquel M.Scarel-Caminaga
Products/Services Used Details Operation
Plasmid DNA Preparation … GFP reporter plasmid (pAcGFP1-1, Clontech Lab. Inc, cat#632497, Mountain View, CA, USA) including a 1 kb fragment of the proximal human IL8 gene promoter was obtained commercially (GenScript Company) … a Construct synthesized by GenScript. b … Get A Quote

摘要

Objective The aim of this study was investigate the functionality of ATC/TTC (Hap-1) and ATT/TTC (Hap-2) Interleukin (IL) 8 gene haplotypes in the response of neutrophils to Gram-negative bacteria associated with periodontitis. Design Neutrophils were isolated by gradient centrifugation from whole peripheral blood of systemically healthy individuals presenting the two IL8 gene haplotypes. Neutrophils were stimulated with P.gingivalis, A.actinomycetemcomitans and PMA/ionomycin, and cytokine gene expression (RT-qPCR) and migration/chemotaxis (boyden chamber assay) were compared according to the presence of Hap-1 or Hap-2 haplotypes. Protein production was also evaluted in the multiplex assay using the mixed popu... More

关键词

Interleukin-8Gram-Negative BacteriaPolymorphism functionalityNeutrophilsLeukocytes