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Custom Vector Construction> | … The miR‑185‑5p and ROCK2 levels were analyzed using the 2‑ΔΔCq method (19). Bioinformatics … The WT‑3'‑UTR of ROCK2 or MUT‑3'‑UTR of ROCK2 was inserted into the pGL3 promoter vector (GenScript Co., Ltd., Nanjing, China) for luciferase reporter experiments … | Get A Quote |
Previous studies demonstrated microRNA-185 (miR-185) as a tumor suppressive microRNA (miRNA) in various types of cancer. The current study aimed to verify this finding in hepatocellular carcinoma (HCC) and explored the downstream channel of miR-185-5p. We detected miR-185-5p and Rho-associated coiled-coil containing protein kinase 2 (ROCK2) mRNA and protein levels by reverse transcription-quantitative PCR (RT-qPCR) and western blotting in HCC tissues and cell lines. Luciferase reporter assay proved the direct relationship between miR-185-5p and ROCK2. Cell migration and invasion were assessed via Transwell assay. miR-185-5p level was reduced in HCC tissues and cell lines. miR-185-5p overexpression impeded migra... More