至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

miR-185-5p targets ROCK2 and inhibits cell migration and invasion of hepatocellular carcinoma.

Oncol Lett. 2019; 
NiuYuexiang,TangGo
Products/Services Used Details Operation
Custom Vector Construction … The miR‑185‑5p and ROCK2 levels were analyzed using the 2‑ΔΔCq method (19). Bioinformatics … The WT‑3'‑UTR of ROCK2 or MUT‑3'‑UTR of ROCK2 was inserted into the pGL3 promoter vector (GenScript Co., Ltd., Nanjing, China) for luciferase reporter experiments … Get A Quote

摘要

Previous studies demonstrated microRNA-185 (miR-185) as a tumor suppressive microRNA (miRNA) in various types of cancer. The current study aimed to verify this finding in hepatocellular carcinoma (HCC) and explored the downstream channel of miR-185-5p. We detected miR-185-5p and Rho-associated coiled-coil containing protein kinase 2 (ROCK2) mRNA and protein levels by reverse transcription-quantitative PCR (RT-qPCR) and western blotting in HCC tissues and cell lines. Luciferase reporter assay proved the direct relationship between miR-185-5p and ROCK2. Cell migration and invasion were assessed via Transwell assay. miR-185-5p level was reduced in HCC tissues and cell lines. miR-185-5p overexpression impeded migra... More

关键词

Rho-associated coiled-coil containing protein kinase 2,hepatocellular carcinoma,invasion,miR-185-5p,migra