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The RNA helicase DHX33 is required for cancer cell proliferation in human glioblastoma and confers resistance to PI3K/mTOR inhibition.

Cell. Signal.. 2019-02; 
WangHongzhong,YuJunyan,WangXingshun,ZhangYan
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Molecular Biology Reagents … The authenticity of cell lines in our study was verified by DNA sequencing using the Applied Biosystems AmpF/STR Identifier kit in 2017 and 2018 … 2.4. Quantitative PCR. The primers used in this study were purchased from GenScript as shown previously [18,19] … Get A Quote

摘要

Human Glioblastoma is one deadly disease; the median survival time is reported to be 13.9 months after treatment. In the present study, we discovered that DHX33 is highly expressed in 84% of all Glioblastoma multiforme (GBM). Knockdown of DHX33 led to significant reduced proliferation and migration in glioblastoma cells in vitro and in vivo. Mechanistically, DHX33 regulated a set of critical genes involved in cell cycle and cell migration to promote glioblastoma development. Additionally, DHX33 was found to be induced by inhibitors of PI3K and mTOR whose activation has been detected in 50% of glioblastoma. Overexpression of wild type DHX33 protein, but not the helicase dead mutant, confers resistanc... More

关键词

DHX33,Glioblastoma,PI3K/Akt/mTOR,RNA heli