Phenotypical characterisation of a putative ω-amino acid transaminase in the yeast Scheffersomyces stipitis.

Phylogenetic analysis of class III transaminases in the budding yeasts Lachancea kluyveri， Saccharomyces cerevisiae and Scheffersomyces stipitis identified a hitherto uncharacterised Sch. stipitis transaminase encoded by the PICST_54153 gene， which clustered with previously described γ-amino butyric acid (GABA) and β-alanine transaminases. Deletion of the PICST_54153 gene in Sch. stipitis resulted in a complete loss in the utilisation of β-alanine and β-ureidopropionic acid as nitrogen sources， while growth on 1，3-diaminopropane displayed a significant lag phase compared to the wild-type control. It was therefore concluded that the Sch. stipitis PICST_54153 gene likely encodes a β-alanine transaminase. However， minor growth defects when 1，4-diaminobutane or 1，5-diaminopentane was provided as the nitrogen source suggested that the Picst_54153 transaminase may also participate in the catabolism of other diamine-derived ω-amino acids. Unexpectedly， the ∆picst_54153 deletion mutant failed to grow on solid minimal medium in the presence of 5 mM β-alanine even if a preferred nitrogen source was provided.