Molecular characterization of ecdysis triggering hormone and its receptor in citrus red mite (Panonychus citri).

Neuropeptide ecdysis triggering hormone (ETH) plays crucial roles in invertebrates by activating a G protein-coupled receptor (GPCR)， the ecdysis triggering hormone receptor (ETHR)， which has been intensively investigated in Hexapoda. However， the molecular characterization of ETH and ETHR in Chelicerata remains unknown. In this study， we identified and characterized the full-length cDNA of ETH and ETHR in citrus red mite Panonychus citri (McGregor). Phylogenetic analysis indicated that PcETHR was closely related to the insect ETHR subtype B. A calcium mobilization-based functional assay showed that PcETH activated the PcETHR in a dose-dependent manner. Furthermore， a sharp upregulation of PcETH before ecdysis was observed by quantitative real-time PCR (qRT-PCR)， and it revealed a tight correlation of ETH signaling and the molting process. These are the first insights into the molecular characterization of ETH and its receptor in mites as P. citri. Our work provides basic information of the ETH and ETHR in P. citri and lays a solid foundation for further physiological functions to better understand the ETH signaling system in mites.