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Electrochemical Detection of Escherichia coli from Aqueous Samples Using Engineered Phages

Analytical Chemistry. 2017; 
Danhui Wang, Juhong Chen, and Sam R. Nugen
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Custom Vector Construction Briefly, the lacZ construct was synthesized in a pUC57 plasmid containing lacZ operon from GenScript (Piscataway, NJ). Get A Quote

摘要

In this study, an enzyme-based electrochemical method was developed for the detection of Escherichia coli (E. coli) using the T7 bacteriophages engineered with lacZ operon encoding for beta-galactosidase (β-gal). The T7lacZ phages can infect E. coli, and have the ability to trigger the overexpression of β-gal during the infection of E. coli. The use of the engineered phages resulted in a more sensitive detection of E. coli by: (1) overexpression of β-gal in E. coli during the specific infection; (2) release of the endogenous intracellular β-gal from E. coli following infection. The endogenous and phage-induced β-gal was detected using the electrochemical method with 4-aminophenyl-β-galactopyranoside (PAPG... More

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