Low antigen dose in adjuvant-based vaccination selectively induces CD4 T cells with enhanced functional avidity and protective efficacy
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| Peptide Synthesis |
The cluster peptide, PCLUS6.1-P18 (GenScript, Piscataway, NJ), containing a H2d -restricted helper epitope (underlined) in the PCLUS6.1 part of the peptide (DRVIEVVQGAYRAIRHIPRRIRQGLER) and the immune dominant H2-Dd –restricted P18-I10 (underlined) contained within P18 (RIQRGPGRAFVTIGK) (23) were used as vaccine Ags. Doses of PCLUS6.1-P18 are indicated in the figures and legends. The molecular mass = 4.9 kDa; hence, a vaccine dose of 1 nmol per mouse corresponds to ∼4.9 mg. For the IL-15–KO study, mice were immunized with 50 mg of the hepatitis B core Ag aa 128–140 (TPPAYRPPNAPIL; GenScript) (24) formulated in CAF09, as described above. |
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