New insights into cooperative binding of homeodomain transcription factors PREP1 and PBX1 to DNA
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| Gene Synthesis |
The DNA template of PREP1 HD in which the four residues K231, K233, K234, and K235 were mutated to alanine was purchased from Genscript Corporation (Piscataway, NJ) and showed the following sequence: 5′CAGCTTCAGTTACAGTTAAACCAAGATCTCAGCATCTTGCATCAAGATGATGGTTCATCTAAGA ACAAGAGGGGCGTCCTGCCAAAGCATGCCACGAACGTGATGCGGTCCTGGCTCTTCCAGCACATCG GGCATCCCTACCCAACAGAGGATGAGAAAAAACAGATTGCTGCTCAGACAAATTTGACACTACTCC AAGTCAACAACTGGTTCATCAATGCCAGAAGACGAATTCTTCAGCCAATGTTGGATTCAAGTTGT TCAGAGACCCCCGCAACAGCGGCAGCAACTGCTCAGAACCGGCCAGTTCAGAGG 3′. |
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