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Enzyme characteristics of pathogen-specific trehalose-6-phosphate phosphatases

Scientific REPORTS. 2017;

Megan Cross, Siji Rajan, Janine Chekaiban, Jake Saunders, Chloe Hamilton, Jeong-Sun Kim, Mark J. Coster, Robin B. Gasser & Andreas Hofmann

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Peptide Synthesis	Codon-optimised expression constructs of the TPP genes from A. ceylanicum (edited sequence of gb:EYC23728.1; see Supplementary Information S1), B. malayi (gb: XM_001893174.1; gene: Bma-gob-1), T. canis (gb:KHN76157.1), M. tuberculosis (gb:CP007299) and S. maltophilia (gb:CCH13862) were obtained from GenScript (USA), ligated into the vector p11 (obtained from The Biodesign Institute, Arizona State University, USA) via Nde I and Bam HI restriction sites, resulting in protein constructs with an N-terminal fusion peptide (MGSSH6SSGRENLYFQGH)	Get A Quote